Periasamy
Sakthidhasan, Ph.D.
About Me
I am Dr. Periasamy Sakthidhasan, a dedicated researcher and laboratory technical assistant with a Ph.D. in Plant Biotechnology from Bharathidasan University, Tamil Nadu, India. My extensive background in molecular biology, coupled with a passion for scientific exploration, has led me to specialize in the isolation, purification, and identification of macromolecules. My research focuses on DNA, RNA, and protein analysis for mutation detection and disease control in plants, animals, and humans.
Research Highlights
Throughout my academic and professional journey, I have engaged in cutting-edge research that bridges the gap between traditional botanical studies and modern biotechnological applications. My doctoral thesis on the phylogenetic analysis and anticancer activity of Mallotus philippensis has provided valuable insights into the bioactive compounds present in this species. The study involved advanced techniques such as GC-MS analysis, size exclusion chromatography, and LC-MS/MS, culminating in significant findings on the cytotoxic properties of specific proteins.
Professional Expertise
In my current role as a Laboratory Technical Assistant, I bring a wealth of experience in operating and managing sophisticated analytical instruments. My proficiency includes:
# Chromatography: High Performance Liquid Chromatography (HPLC), High-Performance Thin-Layer Chromatography (HPTLC)
# Spectroscopy: UV-Vis Spectroscopy, FTIR, Spectrofluorometry
# Microscopy: Confocal Laser Scanning Microscopy, Fluorescent Microscopy
# Molecular Techniques: qRT-PCR, Two-Dimensional Electrophoresis, LC-MS/MS
Technical Skills
$ Microbial Techniques: Isolation and culture of bacteria and fungi, antimicrobial studies
$ Cell Line Culture: Proficient in maintaining and experimenting with various cell lines
$ Genetic Techniques: DNA, RNA, and protein isolation and electrophoresis
$ Metabolite Analysis: Secondary metabolite purification
Testimonials
Dr. Sakthidhasan's expertise in molecular techniques and his dedication to research excellence are truly commendable. His work in phytochemical analysis has significantly advanced our understanding of plant
bioactive compounds
– Dr. T. SENTHIL KUMAR
Proficiency with advanced analytical instruments like HPLC, HPTLC, Spectroscopy and qRT-PCR has been invaluable in our laboratory. His meticulous approach and problem-solving skills set him apart
- Dr. K. EMMANUVEL RAJAN
Leadership and organizational skills during our collaborative works were outstanding. He consistently delivered high-quality outcomes and fostered a productive research environment
- Dr. N. THAJUDDIN
Confocal Laser Scanning Microscope
Zeiss LSM 710 - Laser Scanning Confocal Microscope Workstation, showcasing an exemplary sample image of Drosera burmannii pollen grain stained with fluorescein diacetate.
High Performance Liquid Chromatography
Waters HPLC system equipped with a 2545 Quaternary Gradient Module, 2998 Photodiode Array Detector with fraction collector II powered by Empower II software
Liquid Chromatography Mass Spectrometer
Thermo Scientific LTQ XL Ion Trap (Heated Electro Spray Ionization & Atmospheric Pressure Chemical Ionization) Mass Spectrometer with UHPLC System
Real-Time PCR System
Applied Biosystems™ 7500 Real-Time High-Speed Thermal Cycling system with StepOne software v2.x
Fourier Transform Infrared Spectroscopy
PerkinElmer - Spectrum Two FT-IR Spectrometer with Spectrum 10 software